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Journal of Basic Research in Medical Sciences، جلد ۴، شماره ۳، صفحات ۳۹-۴۱
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Evaluating the serological applications of Toxoplasma gondii rhoptry protein 1 (ROP1) antigen |
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| چکیده انگلیسی مقاله |
Introduction: Toxoplasma gondii (T. gondii) is distributed worldwide and infects most species. The serious incidence and severe or fatal injury caused by T. gondii infection clearly indicates the necessity for the event of a vaccine. The current study goals were to evaluate serological applications of Toxoplasma gondiirhoptry protein 1 (ROP1) antigen. Materials and methods: We created a polymer vaccine by using the eukaryotic plasmid, pROP1. Purification by one-step metal affinity chromatography allowed recovery of milligram amounts of purified recombinant proteins per liter of culture. The quality of this matter for diagnosing of human infections was provided and tested on 77 serum samples which were obtained during routine diagnostic tests. A panel of 20 serum samples from patients with acute toxoplasmosis was compared to a panel of 35 serum samples from individuals with chronic toxoplasmosis. Results: Results of the study indicated that antibodies detected from patients with acute and chronic infections were 96% and 17%, respectively, by using of pROP1 recombinant antigen. Conclusion: According to the present study an immunoglobulin G antibody against ROP1 antigen is made throughout the acute stage of toxoplasmosis infection, but not in the chronic phase of toxoplasmosis. |
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| کلیدواژه‌های انگلیسی مقاله |
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| نویسندگان مقاله |
فاطمه کشاورزی | fatemeh keshavarzi
department of biology, sanandaj branch, islamic azad university, sanandaj, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
پرویز اشتری | parviz ashtari
radiation application research school, nuclear science amp;amp; technology research institute, tehran, iran
پیروز ابراهیمی | pirooz ebrahimi
sapienza university of rome, yas women hospital, tehran
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| نشانی اینترنتی |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-2&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-332462.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Genetics |
| نوع مقاله منتشر شده |
پژوهشی |
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