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Journal of Basic Research in Medical Sciences، جلد ۵، شماره ۲، صفحات ۲۷-۳۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method |
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| چکیده انگلیسی مقاله |
Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI). Results: Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin. Conclusion: A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method. |
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| کلیدواژه‌های انگلیسی مقاله |
Antibiotic Resistance, BCA gene, Colony-PCR, GBS |
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| نویسندگان مقاله |
اعظم خانی daramroodi | aazam khani daramroodi
department of biology, kurdistan science and research branch, islamic azad university, sanandaj, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
فاطمه کشاورزی | fatemeh keshavarzi
department of genetics, sanandaj branch, islamic azad university, sanandaj, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
فرشید رییسی | farshid raissi
department of pathology, faculty of medicine, kermanshah university of medical science, kermanshah, iran
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| نشانی اینترنتی |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-5&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-572375.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Microbiology |
| نوع مقاله منتشر شده |
پژوهشی |
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