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Journal of Basic Research in Medical Sciences، جلد ۴، شماره ۳، صفحات ۳۴-۳۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Isolation and purification of HLA-DR antigen from Daudi cell line by immunoaffinity chromatography |
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| چکیده انگلیسی مقاله |
Introduction: The major histocompatibility complex (MHC) is a group of cell surface proteins that are essential for recognizing foreign molecules in human and other mammals. The physiologic function of MHC molecules is the presentation of peptides to T cells. In this study, we evaluated the purification of a class II MHC molecule (HLA-DR) from a human Burkitt′s lymphoma cell line; Daudi. Materials and methods: We described a simple procedure for purifying human HLA molecules from the cells lysate. As a representative model, HLA-DR was purified from Daudi cell line. The cell membrane was solubilized by a buffer contained NP-40 detergent. Subsequently, the isolation of the membrane antigen was carried out by affinity chromatography method using mouse anti-human HLA-DR monoclonal antibody. The size and the specificity of the purified antigen were determined by Bradford and ELISA methods, respectively. Results: The purified HLA antigen was obtained in approximately 20-30 micrograms in each run of chromatography. Additionally, ELISA method demonstrated the HLA-DR specificity of the purified protein. Conclusion: The results indicated that affinity purification of HLA-DR antigen by means of specific monoclonal antibody is a simple and fast procedure for obtaining the purified antigen. |
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| کلیدواژه‌های انگلیسی مقاله |
HLA-DR, Affinity chromatography, ELISA |
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| نویسندگان مقاله |
زهرا خیاطی | zahra khayyati
ibto bldg, hemmat. exp.way. next to the milad tower, tehran
فاطمه یاری | fatemeh yari
ibto bldg, hemmat. exp.way. next to the milad tower, tehran
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| نشانی اینترنتی |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-212-3&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-332461.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Immunology |
| نوع مقاله منتشر شده |
پژوهشی |
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