Journal of Basic Research in Medical Sciences، جلد ۱، شماره ۴، صفحات ۷-۱۳

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چکیده فارسی مقاله
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عنوان انگلیسی Follicular fluid the best medium of Maturation, fertilization and development of immature oocytes
چکیده انگلیسی مقاله Introduction: In vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment has not been reported yet. Present study was designed to assess the effect of heated human follicular fluid (hHFF), which is similar to in vivo environment for oocyte, on the maturation and fertilization potential of mouse immature oocytes. Materials and methods: Healthy female mice, aged 4-6 weeks, were sacrificed via cervical dislocation and their ovaries were extracted under sterile conditions. After washing, the separated immature oocytes were divided into three groups: In the first group, 236 immature oocytes were placed in culture medium contained DMEM, HCG, FSC 25%, and rFSH. In the second group, 229 immature oocytes were put in culture medium contained 100% hHFF. In the third group, 255 immature oocytes were placed in culture medium contained DMEM, HCG, rFSH, and 25% hHFF. Immature oocytes were placed in an incubator for 24 hours. Then, the stages of oocyte maturation were assessed by invert microscope and mature oocytes in each group were transferred to sperm-contained drops. After 24 hr, rate of two-cell embryos was recorded using invert microscope. Data was analyzed by Chi square test. Results: Maturation rate of oocytes in the second group (87.8%) was significantly higher than first (64.9%) and third (63.2%) groups (p< 0.0005). The difference between first and third groups was not statistically significant (P< 0.2). The formation rate of two-cell embryo in the second group (82.1%) was higher than first (50.2%) and third (54.3%) groups (p< 0.002 and P< 0.01, respectively). Conclusion: it seems hHFF could improve in vitro maturation and fertility potential of immature oocytes and consequently the formation rate of two-cell embryos in mice, in comparison with DMEM even supplemented with 25% hHFF.
کلیدواژه‌های انگلیسی مقاله Follicular fluid, Maturation, fertilization, oocytes

نویسندگان مقاله حجت اله عباس زاده | hojjat allah abbaszadeh


رضا ماستری فراهانی | reza mastery farahani


محسن noorozian | mohsen noorozian


مریم سادات خرمگاه | maryam sadat khoramgah


اردشیر معیری | ardeshir moayeri


علی اصغر کرامتی نیا | ali asghar keramati nia


محمد حسن کریم فر | mohammad hassan karimfar



نشانی اینترنتی http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-96-1&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-120474.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Medical Anatomy
نوع مقاله منتشر شده پژوهشی
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