Journal of Basic Research in Medical Sciences، جلد ۱، شماره ۴، صفحات ۳۴-۴۱

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عنوان انگلیسی Using transient transfected Chinese hamster ovary (CHO) cells by pET28a-LIC-NTF4 in gene therapy
چکیده انگلیسی مقاله Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced prenatally, the capability of growing new neurons from neural stem cells will be preserved by parts of the adult brain. This process is known as neurogenesis. Neurogenesis is stimulated and controlled by neurotrophins. Materials and methods: The recombinant plasmid transformed to E.coli ´ cell and colonies that contain plasmid were selected by Colony PCR. Enzyme digestion and sequencing were monitored to approve the accuracy of extracted plasmid of the clones. Results: Plasmid was verified correctly. Based on RT-PCR and western blotting, the transcription of NTF4 gene and the expression of NTF4 protein after transfection were proved. Conclusion: Plasmid was correctly constructed, CHO Cells were successfully transfected by transfection, and protein could be properly expressed. The results provided a solid foundation for the studies in the area of the transplantation of gene-modified CHO Cells to further spinal cord regeneration.
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نویسندگان مقاله محمد حسن کریم فر | mohammad hassan karimfar

رضا ماستری فراهانی | reza mastery farahani

محسن noorozian | mohsen noorozian

مریم سادات خرمگاه | maryam sadat khoramgah

علی اصغر کرامتی نیا | ali asghar keramati nia

اردشیر معیری | ardeshir moayeri

هادی عظیمی | hadi azimi

حجت اله عباس زاده | hojjat allah abbaszadeh

نشانی اینترنتی
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Medical Anatomy
نوع مقاله منتشر شده پژوهشی
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