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Journal of Basic Research in Medical Sciences، جلد ۸، شماره ۱، صفحات ۴۱-۴۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Evaluation of the effect of PLGA-PAA nano-encapsulated Hydroxytyrosol on inhibiting the colorectal cancer cell line HT-29 and underlying mechanism of action |
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| چکیده انگلیسی مقاله |
Introduction: Chemotherapy was known as a potential approach for colon cancer therapy. Polymer-based nanocarriers prolong the circulation time of chemotherapeutic drugs, therefore anti-tumor drugs can passively accumulate in the malignant tumor position through the improved permeability and retention effect. The aim of the present study was to investigate anticancer potency of biodegradable and pH-sensitive nano-encapsulated Hydroxytyrosol in HT-29 cancer cell line and the potential molecular mechanism of action of Hydroxytyrosol. Materials and Methods: The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hydroxytyrosol was synthesized, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was performed to evaluate the anti-proliferative and anti-tumor effects of both free and nano-encapsulated Hydroxytyrosol. The relative expression of colorectal cancer associated-1 (COCA1) gene was investigated by quantitative Real-Time PCR (qRT-PCR). Results: We observed that free and nano-encapsulated Hydroxytyrosol significantly decreased the viability of HT-29 cancer cells. Moreover, the cytotoxic effect of nano-encapsulated Hydroxytyrosol on HT-29 cancer cells was significantly more than that of free Hydroxytyrosol. Also, the COLCA1 gene expression was up-regulated significantly in HT-29 cancer cells treated with either free or nano-encapsulated Hydroxytyrosol. Conclusion: Generally, we showed that the anticancer potency of Hydroxytyrosol was significantly increased by a biodegradable and pH-sensitive nanoparticle. However, further studies on animal models seem necessary. |
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| کلیدواژه‌های انگلیسی مقاله |
Colorectal cancer, PLGA-PAA copolymer, Hydroxytrylosol, COLCA1 gene |
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| نویسندگان مقاله |
| Masoud Heidari
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
| Mohammadali Hosseinpourfeizi
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
| Nasrin Seyyed Sani
Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
| Narges Dastmalchi
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
| Mohammad Saeid Moradzadeh Tehrani
Department of Molecular Biology, Islamic Azad University, University in Arsanjan, Iran
| Morteza Soleyman-Nejad
Ariagene Medical Genetics Laboratory, Mahmoudnejad Ave, Qom, Iran
| Reza Safaralizadeh
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
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| نشانی اینترنتی |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-500-1&slc_lang=en&sid=1 |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-2473767.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Toxicology |
| نوع مقاله منتشر شده |
پژوهشی |
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