Journal of Basic Research in Medical Sciences، جلد ۷، شماره ۴، صفحات ۳۱-۳۵

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عنوان انگلیسی Multilocus Sequence Typing (MLST) of Enterococcus faecalis clinical isolates in Ilam, Iran
چکیده انگلیسی مقاله Introduction: The Enterococcus faecalis (E. faecalis) is the one of the pathogenic bacteria that become famous and considerable in the recent years. Here we tried to do typing the E. faecalis isolates to provide advantageous information that can help us to understand epidemiological communication between the E. faecalis isolates. Materials and methods: One hundred  E. faecalis were isolated from urine samples of Imam Khomeini Hospital, Ilam, Iran. Afterwards, all isolates were confirmed by the phenotyping method and then for more certainty, every isolates were authenticated by PCR analysis of 16sRNA gene. Eventually, all isolates were considered as E. faecalis. For Multilocus Sequence Typing (MLST), 7 housekeeping genes were used to gain MLST scheme for epidemiological study. In addition, to determine various type of E. faecalis pubmlst database was selected and the MLST analysis was done based on recommended instruction by the Results: The disk diffusion results demonstrated that fifty-four out of one hundred isolates were resistant, four isolates were semi sensitive and forty-two isolates were sensitive to vancomycin. So, 90 isolates were MLST. Using seven structural genes and using database, different types of E. faecalis were determined. The MLST results demonstrated that 26 different group and Sequence Types (ST) obtained. Our findings demonstrated that the isolates were from different types. Conclusion:  Accoeding to our results, we couldn't find any epidemic correlation between the isolates. Given that most of these isolates had resistance to vancomycin, they had low clonal correlation with each other and only had few similar STs pattern.
کلیدواژه‌های انگلیسی مقاله E. faecalis, Multilocus Sequence Typing, Iran

نویسندگان مقاله | Farzad Mohammadi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

| Jalil Vand Yousefi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

| Naser Harzandi
Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

| Sobhan Ghafourian
Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran

نشانی اینترنتی
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Microbiology
نوع مقاله منتشر شده پژوهشی
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