Journal of Basic Research in Medical Sciences، جلد ۷، شماره ۴، صفحات ۵۳-۶۲

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عنوان انگلیسی High prevalence of Toxoplasma gondii infection in ovine aborted fetuses in Gilan Province, Iran: Molecular detection and genotype characterization
چکیده انگلیسی مقاله Introduction: Sheep farming is one of the most important economic aspects of the livestock industry in Gilan province, Iran. Toxoplasmosis is a prevalent zoonotic disease and a major cause of abortion, congenital infection, and stillbirth in animals and humans. Previously, it was assumed that sheep are mostly infected with Toxoplasma gondii (T. gondii) after birth. Nevertheless, recent studies have shown that T. gondii congenital transmission is more prevalent than previously speculated. Therefore, determining the genotypes of this parasite in the intermediate host plays an important role in human infections and prevention programs. This cross-sectional study aimed to determine the T. gondii genetic diversity in aborted ovine fetuses during the lambing season (2018-2019) in Gilan province, Iran. Materials and Methods: Molecular detection of T. gondii was performed in 44 brain tissue samples, collected from aborted ovine fetuses, using a nested polymerase chain reaction (nested-PCR) assay, to target the GRA6 gene. Also, the nested-PCR products belonging to the GRA6-positive samples underwent genotyping with the help of Tru1I (MseI) restriction enzyme using the PCR-restriction fragment length polymorphism (PCR-RFLP) method to classify strains into one of the three major lineages of T. gondii. Results: The results revealed infection in 30 (68.18%) out of 44 brain samples of aborted ovine fetuses, based on the nested-PCR assay with GRA6 gene. Also, the PCR-RFLP results demonstrated the predominance of type II T. gondii in all of the isolates. Conclusion: Overall, the present results revealed the high incidence of T. gondii infection through congenital transmission.This is the first molecular detection and genotyping of T. gondii in ovine aborted fetuses in Gilan Province, Iran.
کلیدواژه‌های انگلیسی مقاله Toxoplasmosis, Sheep, Abortion, Genotyping, Iran

نویسندگان مقاله | Mohammad Reza Chaechi Nosrati
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Bahar Shemshadi
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Parviz Shayan
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


| Shahrokh Ranjbar Bahadori
Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University,Gamsar, Iran


| Ali Eslami
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran



نشانی اینترنتی http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-518-1&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-2473778.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Parasitologiy
نوع مقاله منتشر شده پژوهشی
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