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Plant Biotechnology Persa، جلد ۵، شماره ۱، صفحات ۰-۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Application survey of zinc nanoparticles containing Ocimum basilicum leaf aqueous extract on nicotine-induced cell death in PC12: A pre-clinical trial study |
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| چکیده انگلیسی مقاله |
High concentration of nicotine induces cell death in central nervous system which can affect the health of nicotine-exposed people. Some therapeutic properties of Ocimum basilicum leaves and zinc nanoparticles have been investigated but its protective power against neuron cell death not studied yet. Therefore, we studied the protective effect of zinc nanoparticles green-synthesized by Ocimum basilicum leaves aqueous extract in nicotine-treated PC12 cells. The cell viability measured by LDH test. TUNEL test was used to show DNA fragmentation and apoptosis. The Rat inflammatory cytokine assay kit was used to measurement of inflammatory cytokines concentrations. 100 mM of nicotine reduced cell viability, and increased cell cytotoxicity, inflammatory cytokines concentrations, and DNA fragmentation significantly. Zinc nanoparticles green-synthesized by Ocimum basilicum leaves aqueous extract increased cell viability and decreased cell cytotoxicity, inflammatory cytokines concentrations, and DNA fragmentation in high concentration nicotine-treated in PC12 cells, respectively. The main finding of our study was that zinc nanoparticles green-synthesized by Ocimum basilicum leaves aqueous extract suppresses the nicotine-induced cell death in PC12 neuron-like cells. It inactivates the apoptosis process through inflammatory cytokines synthesis. |
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| کلیدواژه‌های انگلیسی مقاله |
Nicotine,Neuroprotective,Zinc nanoparticles,Cell cytotoxicity,PC12 cells |
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| نویسندگان مقاله |
| Seyed Morteza Hosseini
Medicine, Quran and Hadith Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
| Namdar Yousofvand
Department of Biology, Faculty of Science, Razi University, 67149-67346, Kermanshah, Iran
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| نشانی اینترنتی |
http://pbp.medilam.ac.ir/browse.php?a_code=A-10-532-3&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Herbal Drugs |
| نوع مقاله منتشر شده |
پژوهشی |
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