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Journal of Basic Research in Medical Sciences، جلد ۴، شماره ۳، صفحات ۴۲-۴۷
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عنوان فارسی |
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چکیده فارسی مقاله |
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کلیدواژههای فارسی مقاله |
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عنوان انگلیسی |
Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay |
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چکیده انگلیسی مقاله |
Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples were collected from Rasht City rivers and lagoons during 2009-2010. The samples were analysed for Cryptosporidium oocysts using Real Time PCR method. Samples were filtrated through a 1.2µm pore size cellulose nitrate membrane filter and then purified and quantified by Real-time PCR technique. Results: Cryptosporidium oocysts were found in 19 of 30 the samples. Oocyst concentration was ranging from 0.007 to 27 oocysts per liter of the examined waters. Conclusion: The present study showed that the investigated water supplies were contaminated by Cryptosporidium oocyst. This study indicated that in this level of oocysts there is a potential risk of waterborne cryptosporidiosis due to direct or indirect consumption of these waters by humans and animals. Real-time PCR is a technique that provides high sensitivity for detection quantitative purposes. |
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کلیدواژههای انگلیسی مقاله |
Cryptosporidium spp., Water, Iran, Real-time PCR |
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نویسندگان مقاله |
محمدرضا محمودی | mohammad reza mahmoudi department of parasitology, faculty of medicine, guilan university of medical sciences, rasht, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی گیلان (Guilan university of medical sciences)
مژگان بنده پور | mojgan bandepour cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
بهرام کاظمی | bahram kazemi department of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
اسد میرزایی | asad mirzaei department of parasitology, faculty of allied medical sciences, ilam university of medical sciences, ilam, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)
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نشانی اینترنتی |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-58-2&slc_lang=en&sid=en |
فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-332463.pdf |
کد مقاله (doi) |
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زبان مقاله منتشر شده |
en |
موضوعات مقاله منتشر شده |
Parasitologiy |
نوع مقاله منتشر شده |
پژوهشی |
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