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Journal of Basic Research in Medical Sciences، جلد ۵، شماره ۲، صفحات ۸-۱۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Time dependent of epigenetic effect of disulfiram on tumor suppressor gene of RASSF1A in Hela cancer cell line |
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| چکیده انگلیسی مقاله |
Introduction: Cervical cancer is the third most common tumor among women. Surgery, radiotherapy, and chemotherapy are common treatments, however high stage tumors have frequently poor prognosis. Nowadays, the epigenetic reversion introduced as an efficient strategy of treatment of cervical cancer. In the process, inhibitors of DNA methyltransferase (DNMT) induce re-expression of tumor suppressor genes. Among these inhibitors, disulfiram (DSF) has been suggested as non-nucleoside analogous. In this research, we evaluated the epigenetic effect of DSF on demethylation of the tumor suppressor gene, RASSF1A, in Hela cell line. Materials and methods: Hela cells were cultured and treated with different doses from 2.5 to 37.5µM during 24, 48 and 72 hours. MTT assay was carried out to find half maximal inhibitory concentration (IC50). The methylation specific PCR (MSP) assay was applied to evaluate methylation pattern. Results: The IC50 of DSF was determined at the 2.5, 12.5, and 15µM after72 hours. The MSP results showed partial demethylation at mentioned concentrations after 72h but unmethylated band was not observed after 24h. Conclusion: Our findings indicated that, IC50 of DSF exerted a biphasic effect in Hela cell line and at least 72 hours treatment is needed for the epigenetic reversion of DSF on RASSF1Ain Hela cell line. |
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| نویسندگان مقاله |
سیده نغمه noorirad | seyedeh naghme noorirad
department of anatomical science, faculty of medicine, babol university of medical sciences
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
محسن پورقاسم | mohsen pourghasem
department of anatomical science, faculty of medicine, babol university of medical sciences
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
فریده فیضی | farideh feizi
department of anatomical science, faculty of medicine, babol university of medical sciences
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
زینب عابدیان | zeinab abedian
cellular and molecular biology research center, health research institute, babol university of medical sciences, babol, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
معصومه قاسمی | masoumeh ghasemi
cellular and molecular biology research center, health research institute, babol university of medical sciences, babol, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
زهرا بابازاده | zahra babazadeh
department of anatomical sciences, faculty of medicine, babol university of medical sciences, babol, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بابل (Babol university of medical sciences)
نوید ربیعی | navid rabiee
department of chemistry, shahid beheshti university, tehran, iran
سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)
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| نشانی اینترنتی |
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-303-1&slc_lang=en&sid=en |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-569226.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Genetics |
| نوع مقاله منتشر شده |
پژوهشی |
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